Endotoxin-induced microparticle formation is subject to tolerance development in humans
نویسندگان
چکیده
Methods Formation of MP was studied in 8 healthy male subjects exposed to intravenous E. coli endotoxin (LPS; 2 ng/kg body weight) who were re-challenged after 17 ± 11 days. In addition, intravenous vitamin C (25 g) or placebo was administered 3.5 hours after LPS on alternate trial days in random order. Venous blood samples were taken before, 3 h and 6 h after LPS administration. MP were detected using FACS analysis. Leukocyte count and body temperature were measured as positive control. All results are expressed in medians in the unit 10 MP/μL plasma.
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